ABSTRACT
This study was designed to inspect the association between tumor necrosis factor (TNF)-α-308G/A (rsl800629)polymorphism with diabetes mellitus type-2 (DMT2) in Bangladeshi population. Besides, the role ofTNF-α-308G/A in early proliferation of DMT2 has been investigated. The allelic frequency of TNF-α-308G/Aand their association with DMT2 was studied using high resolution melting (HRM) analysis and confirmedusing sequencing. A few demographic risk factors associated with DMT2 were also investigated routinely.The significance of these risk factors was analyzed statistically. We have analyzed 657 individuals who weredistributed into two groups: 330 non-diabetic controls and 327 DMT2 individuals. HRM analysis showsthat 11 individuals bare G/A and 2 bare A/A genotype in DMT2 patients. Within non-diabetic individual,we found only one with G/A genotype. The frequency of TNF-α-308G>A are within the Hardy–Weinbergequilibrium (0.00482) at 95% confidence level. TNF-α-308G>A frequency in two age group based on firsttime diagnosed, we found association with early proliferation of DMT2 with a p-value of 0.008965 in Fisher’stest at 95% confidence level. Our result suggested that the single nucleotide polymorphisms TNF-α-308G>Ais closely associated with DMT2 patients in Bangladeshi population. Besides, presence of TNF-α-308G>Apolymorphism increases the risk of early proliferation of DMT2.